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Drought Tolerance of Robust Tobacco Seedlings in a 1020 Tray

deluxestogie

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Escape from the Tray of the Damned: a seedling drought story

Garden20230705_7089_trayOfTheDamned_before_600.jpg

5 July 2023

Newly germinated seedlings were transferred to cell inserts in 1020 trays in early March 2023. As transplants were put into their respective garden beds, my intentional extras were eventually consolidated into this one remaining 1020 tray.

Purely for the sake of science and the betterment of mankind, I ignored this tray (i.e. never bothered to watered it) from some date in late May until 5 July. It contained my spare transplants, in case I needed to replace any transplant mortality in the garden beds. But by the final week of May, I realized that my transplant mortality for the season was a surprising zero. No need for replacements. No need to continue watering the tray of spares. The 1020 tray remained on a wire shelf in my enclosed back porch, with at least ½ day of direct sunlight through the glass.

Varieties in the tray (There were no Tofta extras.):
  • NB-11 burley
  • Little Yellow
  • Xanthi-Yaka 18a
  • Corojo 99
  • Olor
  • Vuelta Abajo
  • Maryland 609
Since these sorry plants had clearly seen better days—in addition to their not being needed, I decided the time had come to toss them over the back fence. But...but how is there still green showing on many of them? Zero water for 5+ weeks. Inspecting beneath the inserts and the 1020 tray with holes, I saw only bone dry, skeletal appearing roots.

Does the scattered greenery indicate that they could actually recover with the addition of water now? If it does, then that would certainly say something about the drought tolerance of Nicotiana tabacum. (As a bonus, by just adding water, I could postpone having to carry the contents of the tray to its destined grave beyond the back fence.) It's citizen science!

I added 1½ quarts of water to the tray in mid morning. Within 20 minutes, the tray was dry. I added another 1½ quarts. I could almost hear epigenetic whispering, if I held my ear really close.

After 24 hours:
Garden20230706_7095_trayOfTheDamned_24hrs_600.jpg

6 July 2023

After 48 hours:
Garden20230707_7096_trayOfTheDamned_48hrs_600.jpg

7 July 2023

I added water to the upper tray (with holes) as needed, to keep the soil from drying out.

At the end of a week:
Garden20230712_7104_trayOfTheDamned_1wk_600.jpg

12 July 2023

Counts of Surviving Varieties: [count survived / initial count]
  • NB-11 burley: [4 / 4]
  • Little Yellow: [2 / 4]
  • Xanthi-Yaka 18a: [6 / 12]
  • Corojo 99: [0 / 4]
  • Olor: [4 / 4]
  • Vuelta Abajo: [3 / 4]
  • Maryland 609: [3 / 3]
CONCLUSION:
So long as the apical meristem (growth tip) of the plant is still alive, OR if there is at least one node on the stalk still alive (at a leaf axil), the plant will resume growth upon watering. Some of those that didn't make it might still be able to send up a sucker from the root system, though I did not observe that. A question not addressed by this experiment is whether or not there is a specific window of age (post germination) during which a tobacco seedling can survive such an ordeal. The seedlings in the Tray of the Damned were all about 3 months post germination at the start of the drought, and were over 4 months post germination in the final image.

I'm not suggesting that you ever do this with your plants, but the exercise indicates just how drought tolerant tobacco can be, once it has developed a root system and a sturdy seedling stalk.

Bob's Aphorism: Overwatering kills; underwatering builds character.

I could, if I wished, transfer survivors into 3-inch pots, and maintain them as runted plants through the winter—topping them above the lowest leaf each time they grew too tall. Then in the spring, if such a plant is finally transplanted to the garden with at least one leaf node above the ground, it would grow into a relatively normal, primary season plant, with a reasonably normal primary crop of nice leaf. [I demonstrated that a few years ago with small, potted tobacco plants of several varieties, that I did not transplant to the garden until their third season of growth.]

Bob
 

johnny108

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Tobacco drought stress responses reveal new targets for Solanaceae crop improvement​


We propose components of a core metabolic response to drought stress in plants and also show that some major responses to drought stress at the metabolome and transcriptome levels are family specific. We therefore propose that the observed family-specific changes in metabolism are regulated, at least in part, by family-specific changes in transcription factor activity. We also present a list of potential targets for the improvement of Solanaceae drought responses.
 

johnny108

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Gotta agree with Bob.
I just transplanted an (Amazon sourced) "Virginia" seedling into a self-watering bucket with soilless mix and time release fertilizer.
It had been in small 50/50 perlite/vermiculite cup, with a wick going into the same organic fertilizer that grew a hydro-organic Xanthi for me. The wick was just a strip of cotton cloth from an old t-shirt.
After one month- there was almost no root development! Nothing had grown into the wick, which normally happens very quickly with tomatoes and peppers. The perlite/vermiculite was soaked and compacted.
The Xanthi was only hand watered- usually whenever I saw it wilted from neglect, and the root ball is encircling the pot, and growing out the drain holes.
 

deluxestogie

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I could, if I wished, transfer survivors into 3-inch pots
I transferred them into the waste basket beside my clothes drier, where some are still attempting to put out new leaves, despite lack of water. But with used fabric softener sheets surrounding them, at least they will be soft and cuddly, and smell like fresh air.

Bob
 
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