It's been two days, and all my tobacco transplants are alive.
I treat my new seedlings as though they are growing in float beds in a greenhouse, even though they are actually resting on wire shelves at the windows of my enclosed back porch. They tend to get a little over ½ day of direct sunlight through the glass, from the time they are quite tiny. I use no grow lights. I do not pack soil into the inserts, but simply fill them, then scrape away any excess, so the soil mix remains relatively loose. There is always water in the 1020 trays without holes, and whenever the soil in the inserts begins to dry a bit, I add water beneath them, into the 1020 trays with holes. They soak up what's there within a day. I clip the leaves 4 or 5 times prior to transplant.
Without further hardening or drying, or gradual introduction to reality, they go directly from the enclosed porch to the garden bed, with 2 cups of transplant water per plant, to settle the soil around the roots. For me, in my area, this works fairly consistently, so long as I start germination 2 months prior to last frost.
Bob